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What’s new in knowledge zone?<\/p>\n\n\n\n
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- qPCR<\/a><\/li>\n\n\n\n
- Reversed Phase Chromatography<\/a><\/li>\n\n\n\n
- Size Exclusion Chromatography<\/a><\/li>\n\n\n\n
- What are theoretical plates?<\/a><\/li>\n\n\n\n
- PCR reaction<\/a><\/li>\n\n\n\n
- Cytotoxicity tests<\/a><\/li>\n\n\n\n
- How to count cells in Burker chamber?<\/a><\/li>\n\n\n\n
- Protein determination methods<\/a><\/li>\n\n\n\n
- PBS<\/a><\/li>\n\n\n\n
- Fundamentals of electrophoresis<\/a><\/li>\n<\/ol>\n<\/div><\/div>\n\n\n\n
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maj <\/i><\/a><\/div><\/div>\r\n <\/div>\r\n \r\n\r\n- PN<\/dt>
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<\/div>Do You calculate Linearity properly?<\/a>Imagine the following situation. You are testing the linearity of an analytical method, let’s say HPLC. The linearity coefficient is R=0.999, the coefficient of determination is R2 = 0.999. An ideal situation. The criteria are met, the validation is successful. Now all that’s left is to write the report and go home. Nothing could be…<\/div><\/li>\n<\/div>Czy dobrze liczysz swoj\u0105 liniowo\u015b\u0107?<\/a>Wyobra\u017a sobie nast\u0119puj\u0105c\u0105 sytuacj\u0119. Testujesz liniowo\u015b\u0107 metody analitycznej, powiedzmy HPLC. Wsp\u00f3\u0142czynnik liniowo\u015bci R=0,999, wsp\u00f3\u0142czynnik determinacji R^2 = 0,999. Sytuacja idealna. Kryteria spe\u0142nione, walidacja zako\u0144czona sukcesem. Teraz tylko raport i do domu. Nic bardziej mylnego. W naszych danych mo\u017ce by\u0107 ukryty chochlik, kt\u00f3rego na pierwszy rzut oka nie wida\u0107. Pomo\u017ce nam w tym jednak analiza resztkowa.<\/div><\/li>\n![\"\"](\"https:\/\/bioeducator.eu\/wp-content\/uploads\/2025\/12\/Scientist_question_mark-150x150.png\")
<\/div>When to conclude the development of an analytical method?<\/a>How do you know when to stop optimising your analytical method? This question was asked during one of the training sessions I was giving. Although I knew the answer, it still surprised me. Because it’s not obvious at all.<\/div><\/li>\n
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